FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005738752

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005738752
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5667670
Sequences flagged as poor quality0
Sequence length150
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATCGGCG1950.073.846158
TCGGCGG4350.034.758629
TATCGGC4450.032.359557
TTATCGG5000.027.366
ATTATCG10351.8189894E-1213.2173915
TCGTCGC2350.012.86808515-19
CGATCGG1552.5279405E-611.148388130-134
CTCGTCG3100.010.68387115-19
CGTCGCT3351.8189894E-129.88656715-19
CCGATCG1902.849923E-59.09473755-59
CGATCGC2056.952251E-58.42926860-64
CCGGCGA3401.4317684E-67.230-34
GCGATCG2506.879542E-46.912130-134
CGGCGAG4002.6153411E-66.479999530-34
TCCGGCG3951.4860758E-56.197468325-29
CGTTACC4751.0673975E-66.06315815-19
TCGTTAC4901.8579049E-65.87755115-19
GTTACCG4459.165727E-55.501123420-24
CCAGTAC31601.8006904E-75.46835477
CAGTACC32002.3387656E-75.39999968
Produced by FastQC (version 0.11.5)