FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005738771

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005738771
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3274929
Sequences flagged as poor quality0
Sequence length150
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATCGGCG2550.087.529418
TCGGCGG3700.060.324329
TATCGGC4000.055.8000037
TTATCGG4750.045.4736866
ATTATCG7050.032.680855
CCGGCGA3400.013.12941230-34
TCGTCGC1354.7695903E-712.815-19
CGGCGAG3800.012.50526330-34
CGATCGG1407.4109084E-712.342857130-134
TCCGGCG4150.012.14457825-29
GTTACCG4150.012.14457820-24
CGTTACC4150.011.45060315-19
CTCGTCG1409.966081E-611.31428615-19
CCGATCG1152.3101737E-411.26956555-59
GGTTCGT4450.010.67865215-19
AATCCGG5000.010.65625-29
TTACCGA4650.010.52903220-24
CGTCGCT1401.2383232E-410.28571415-19
GAATCCG5000.010.0825-29
GCGATCG1604.3560154E-59.9130-134
Produced by FastQC (version 0.11.5)