FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005738783

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005738783
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6602461
Sequences flagged as poor quality0
Sequence length150
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATCGGCG2900.069.517248
TCGGCGG4450.045.3033689
TATCGGC6000.032.47
TTATCGG6300.028.5714286
ATTATCG11150.016.7892385
CGATCGG2053.8380676E-1011.9414625130-134
TCGTCGC3200.011.70000115-19
TCCGGCG4750.010.00421125-29
CTCGTCG3600.010.015-19
CCGATCG2005.1291245E-69.36000155-59
CGTCGCT4450.09.06067415-19
CGATCGC2806.4337655E-88.74285760-64
CCGGCGA5350.08.07476630-34
CGGCGAG5550.07.78378430-34
GCGATCG2551.0801359E-47.341177130-134
ATCCGGC6850.06.93722725-29
CAGTACC37900.06.83905038
AATCCGG7350.06.66122525-29
CCAGTAC38251.8189894E-126.2117657
TACCGAA7651.8189894E-126.211764320-24