FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005738870

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005738870
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2453954
Sequences flagged as poor quality0
Sequence length150
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATCGGCG1300.088.615398
TATCGGC1850.062.270277
TCGGCGG2250.051.29
TTATCGG2450.044.081636
ATTATCG4450.024.269665
CGATCGG654.303365E-822.153847130-134
CCGATCG601.1753082E-519.19999955-59
TCGTCGC1300.018.83076915-19
CGTCGCT1550.016.72258215-19
CTCGTCG1351.1459633E-1016.015-19
CGATCGC902.3293182E-514.460-64
TCCGGCG1804.3655746E-1113.625-29
CGGCGAG2103.6379788E-1213.02857130-34
GCGATCG1101.528266E-411.781818130-134
CCGGCGA1901.913213E-811.36842130-34
ACCGCGC6003.4243276E-410.81
TACCGAA2353.6870915E-910.41702220-24
CGCGCCC5850.00336080259.8461533
GTTACCG2405.658876E-89.59999920-24
TCGTTAC2457.7767254E-89.40408115-19
Produced by FastQC (version 0.11.5)