FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005738892

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005738892
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5806890
Sequences flagged as poor quality0
Sequence length150
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATCGGCG2400.063.0000048
TATCGGC4450.035.5955057
TCGGCGG4450.033.9775289
TTATCGG6200.022.0645166
CTCGTCG2800.018.51428415-19
TCGTCGC2650.017.3886815-19
CCGATCG1453.4015102E-1014.89655255-59
GCGTCCG3600.001018818514.04
ATTATCG9901.6370905E-1113.0909095
CGTCGCT3750.013.05615-19
CGATCGG1553.0724987E-510.219356130-134
TCCGGCG3800.09.85263225-29
CCGGCGA4051.2732926E-118.53333430-34
CCAGTAC31500.08.4571437
CGGCGAG4600.08.45217430-34
CGATCGC2703.1465988E-68.060-64
CAGTACC31800.07.69811348
GACCTCC41300.07.3220342
ACCGTAA8500.07.28470635-39
GCGATCG2404.3136204E-47.2000003130-134