FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005739129

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005739129
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4869173
Sequences flagged as poor quality0
Sequence length150
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATCGGCG1252.8737122E-740.328
TCGGCGG3901.1140891E-414.769239
TATCGGC3100.0061234613.9354847
CCGATCG1801.5044972E-59.655-59
TCGTCGC2951.6298145E-99.27457615-19
CTCGTCG3555.7843863E-108.51831115-19
CGTCGCT3554.4536137E-87.707042715-19
AGTACCT31404.2127795E-95.96178349
CAGTACC26801.5138048E-65.64179138
GACCTCC39855.800757E-95.2396492
ACCGTAA6604.4271474E-75.23636435-39
CCAGTAC30452.8297582E-65.20197067
CCGTAAA7103.7103382E-75.070422635-39
CCGAGTA23008.593579E-45.0086962