FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005739199

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005739199
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7448463
Sequences flagged as poor quality0
Sequence length150
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATCGGCG2850.055.5789458
TCGGCGG5250.031.5428569
TATCGGC5750.027.5478277
TTATCGG6250.021.8886
CGATCGG2108.333882E-810.285714130-134
ATTATCG14006.330083E-109.7714285
TCGTCGC4151.8007995E-107.98072315-19
CTCGTCG5002.1827873E-117.48815-19
CCGGCGA5102.582965E-107.058823630-34
CGTCGCT5001.2387318E-96.911999715-19
TCCGGCG5704.5292836E-106.56842125-29
GCGATCG3500.0049440686.1714284
CGGCGAG6804.285539E-95.71764730-34
CAGTACC43152.6193447E-105.33951338