FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005739219

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005739219
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4988239
Sequences flagged as poor quality0
Sequence length150
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATCGGCG1900.068.2105268
TCGGCGG3050.042.4918029
TATCGGC3950.032.8101277
TTATCGG5700.024.0000026
ATTATCG8000.016.1999995
CGATCGG1407.411436E-712.342857130-134
CCGGCGA3606.7448127E-98.030-34
CGGCGAG4355.820766E-117.94482830-34
GCGATCG2004.914752E-47.9199996130-134
ATCCGGC5655.0931703E-116.881415425-29
TCCGGCG4403.715104E-86.872727425-29
GTTACCG4402.693323E-76.545454520-24
CGTTACC4657.2917646E-76.193548715-19
TCGTTAC4851.5479272E-65.93814415-19
AAGTCGG5801.1172269E-65.462069140-144
TACCGAA5851.3168792E-65.41538520-24
GCGGGTT6751.257431E-75.333333510-14
AATCCGG6258.300758E-75.299225-29
Produced by FastQC (version 0.11.5)