FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005739272

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005739272
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5739315
Sequences flagged as poor quality0
Sequence length150
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATCGGCG1850.062.270278
TCGGCGG4450.024.269669
TATCGGC4900.023.5102047
TTATCGG5403.6379788E-1220.06
ATTATCG10407.239032E-810.3846165
TCGTCGC1851.8966621E-610.11891915-19
TCCGGCG3652.1145387E-47.89041148
CTCGTCG2601.372705E-47.215-19
CCGGCGA3358.833877E-66.87761230-34
CGTCGCT2752.7336716E-46.80727315-19
AAGTCGG5203.117876E-55.2615385140-144
AATCCGG5755.497146E-65.259130525-29
CGGCGAG4853.3256528E-45.047422430-34
CCCAAGT81550.05.03249551