FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005739426

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005739426
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2142443
Sequences flagged as poor quality0
Sequence length150
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATCGGCG756.002665E-1176.88
ATGCGCG605.11806E-448.02
TCGGCGG1355.2520954E-737.3333329
TATCGGC1607.161239E-527.07
GCGAGTA1500.001556294624.01
GCGACAT1750.003809799220.5714281
GCGTAGG1850.005254823219.459461
TTATCGG2000.00824121818.06
GCGAAGA3153.6982598E-416.01
TCGTCGC901.3168665E-616.015-19
CCGTAAT3200.007553815413.52
CGTCGCT1151.6663562E-512.52173915-19
CTCGTCG1253.913923E-511.5215-19
CGAAAAT4500.005436097711.22
CGAAAAA5400.001728969210.6666663
CGAAGAA5450.001866884210.5688082
CGTGTTG4850.00948200910.3917513
AAGTCGG2901.0192871E-67.944828140-144
CAGTACC12255.4553576E-47.0530618
CGGTTTG3350.0029472386.4477612
Produced by FastQC (version 0.11.5)