FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005739427

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005739427
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2142443
Sequences flagged as poor quality0
Sequence length150
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATCGGCG950.090.9473658
TCGGCGG1750.049.3714269
TATCGGC2050.042.1463437
TTATCGG2700.034.6666646
ATTATCG4205.820766E-1122.2857155
CCGGCGA1501.2611781E-712.480000530-34
TCCGGCG1851.2898454E-811.67567525-29
TAAGTCG1152.3100308E-411.269566140-144
GTTACCG1952.806155E-811.07692220-24
CGGCGAG1759.289961E-710.69714330-34
AATCCGG2201.7316215E-69.16363625-29
TCGTTAC2056.951003E-58.42926815-19
CGTTACC2252.2707922E-58.3215-19
CCCTAGT8850.00129191668.1355921
AAGTCGG3206.364462E-88.099999140-144
CCGAATC2509.703921E-68.06420-24
ATCCGGC2353.914071E-57.965957625-29
TACCGAA2201.5814556E-47.854545620-24
GAATCCG2558.6181745E-46.776470725-29
GGTTCGT2558.6181745E-46.776470715-19
Produced by FastQC (version 0.11.5)