FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005739504

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005739504
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3293533
Sequences flagged as poor quality0
Sequence length150
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATCGGCG1201.8189894E-1260.08
TCGGCGG2502.6393536E-928.89
TATCGGC2604.0126906E-927.6923087
TTATCGG3301.2177716E-619.6363646
GCGACCA2600.001898787716.6153851
ATTATCG4702.07037E-615.3191495
GCGAGGA5702.1293062E-411.3684221
CGAGTGT5150.001164368411.1844652
GCGAAAA4600.00640342210.9565221
TCGTCGC950.00812908210.61052615-19
CTCGTCG1350.00101286519.615-19
CGAGAAG6800.00108314789.5294122
GCGCAGG7653.02765E-49.4117651
CGTCAAA6250.00580084049.2162
TCGTTAC2151.2816363E-58.70697715-19
TCCGGCG2354.2271495E-68.57872325-29
GCGAGGC7750.00355594358.3612911
CGCAGGC8000.0047342648.12
CGAGGAG8100.0052938318.04
AGCGTGG9150.00179556877.8688533
Produced by FastQC (version 0.11.5)