FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005739618

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005739618
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7870534
Sequences flagged as poor quality0
Sequence length150
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATCGGCG2700.040.0000048
CGCGATT1804.965612E-628.03
TCGGCGG4650.024.7741959
TTATCGG6401.8189894E-1218.06
TATCGGC5752.0372681E-1017.5304367
GCGACAA8053.163659E-711.6273291
GCGAGTA4750.00812845310.6105251
CGCATTA6406.213137E-410.1253
GCGAAAG11402.5580448E-810.1052641
GCGAGCT9402.329436E-69.9574471
GCGTAGA6557.687036E-49.8931291
GCGCAAA7502.4826158E-49.61
CGAGCTA6750.00101274129.62
GCGAAGA12056.0323146E-89.5601661
GCGAACA7653.0288487E-49.4117651
CGAAAGT11553.3391188E-79.350652
CGAAGAG16309.795258E-98.3926382
GCGAAAA13202.2825116E-68.1818181
TCGTCGC3258.272582E-87.975384715-19
CGAGGAT11954.8308437E-57.8326362
Produced by FastQC (version 0.11.5)