FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005739638

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005739638
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5779782
Sequences flagged as poor quality0
Sequence length150
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATCGGCG1307.825292E-944.307698
TCGGCGG2354.604226E-827.5744679
TATCGGC3204.1706645E-415.757
GCGAAAA8755.655238E-912.3428571
GCGAGGA9151.0977601E-811.8032781
CGAACAT7451.5717815E-611.5973162
GCCGAAA6857.830766E-611.5620431
GCGCAAA5702.1298366E-411.3684211
GCGATAT5200.001262543511.0769221
CGAAAGT8951.4077908E-59.6536312
TCGTCGC1656.104649E-59.615-19
TCGCAAA7602.835641E-49.4736841
GCGAAAT6900.00123809179.3913051
GCGAAGA7350.0022011958.8163271
CCGAAAG10005.205115E-58.6399991
CGCAAAA9753.7429397E-48.1230772
CCGAAGT8300.006590787.8072291
CGAAAAA15853.0218564E-56.81388042
GCGAGGG12950.00102823776.67181441
CGAGAAA13900.00228611386.21582752
Produced by FastQC (version 0.11.5)