FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005739657

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005739657
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6569391
Sequences flagged as poor quality0
Sequence length150
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATCGGCG2250.051.28
TATCGGC4500.027.27
TCGGCGG4800.024.09
TTATCGG5600.021.8571436
ATTATCG10100.014.9702975
CCGATCG1552.5279805E-611.14838855-59
TCGTCGC2354.28392E-79.19148915-19
CTCGTCG2805.276759E-67.71428615-19
CCGGCGA3707.2372313E-77.00540530-34
CGATCGC2150.0086438076.697674360-64
TCCGGCG4105.532238E-76.67317125-29
CGTCGCT3356.5110085E-56.447760615-19
CGAGTAG32352.0372681E-106.23183873
CCGAGTA32752.764864E-106.1557252
CGGCGAG4501.7673736E-55.7630-34
AAGTCGG8756.730261E-115.4308567140-144
CCCGAGT43002.382876E-105.35813951
CGTTACC6203.978088E-65.10967815-19