FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005739770

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005739770
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6343317
Sequences flagged as poor quality0
Sequence length150
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATCGGCG2105.3048643E-727.4285728
GCGACGT1650.002709053721.8181821
TCGGCGG4601.6556423E-615.6521759
CGACGTG2900.003933348714.8965512
CCGAAAG9751.2732926E-1113.2923081
TATCGGC4100.002712153612.2926837
CGCGAGC4705.4084277E-412.2553192
GCGAAGA9602.2320819E-811.251
CGAAAGT9201.4978832E-710.9565232
CGAGCTA4750.00812816110.6105251
TTATCGG4850.00948544210.3917526
GCGAGTA4850.00948544210.3917524
CCGAAAA10558.927964E-810.2369671
GCGAACA6750.00101268419.61
GCGAGTT6400.00705033969.03
CGAGCTT7800.00376946648.3076922
CGAAAAA14801.5743171E-78.270272
GATTCGA8000.0047354938.0999995
CGCAAAG11653.5168192E-58.0343351
CGAGAGG13653.6805795E-67.9120882
Produced by FastQC (version 0.11.5)