FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005739794

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005739794
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7345363
Sequences flagged as poor quality0
Sequence length150
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATCGGCG2150.043.5348858
TATCGGC4307.8216544E-1121.7674437
CGTCGTA1750.003810666720.5714282
TCGGCGG4904.5474735E-1019.1020419
TTATCGG6051.9540475E-613.0909096
GCGAATA5107.5514916E-512.7058831
CGAGCTA6454.003403E-612.2790692
GCGACAT6002.5780739E-512.01
GCGAAAT9651.891749E-911.9378231
GCGACTA4800.00878464510.51
GCGATAA6456.6738913E-410.0465121
GCGCAGA10801.2572491E-710.01
CCGAGCT11001.642984E-79.8181821
TCGTCGC2354.0887244E-89.80425515-19
CGCGCAC5950.00386796369.6806732
GCGAGCA9553.0307552E-59.047121
GCGAACA6400.0070505619.01
CGAAAGT12055.933316E-68.3651452
CGAAGTA7900.00422850348.2025322
CCGAAGT11452.8318727E-58.1746731