FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005739846

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005739846
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5139543
Sequences flagged as poor quality0
Sequence length150
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATCGGCG1307.825292E-944.3076938
TATCGGC3153.0895535E-822.8571437
TCGGCGG2908.769392E-619.862079
TTATCGG3707.111543E-515.5675686
GCGATTA5501.0545236E-513.0909091
CTAGCGT4350.00422304911.5862062
GCGAAAT5702.1297488E-411.3684221
CGAAAGT7802.7293736E-611.0769222
CGAAGTA5400.00172967910.6666672
CGAGCAT6254.9937126E-410.3682
CCGAAAA9803.9640036E-69.551022
CGAGGAT7000.00141183829.2571432
CCGAAGA7400.00234081328.7567571
ATTATCG7400.00234081328.7567575
CCGAAAG9201.9997169E-48.6086961
GCGAAGA7550.00280778278.5827821
CCGAAGT8559.187335E-48.4210531
CGAGAGT8000.00473518258.0999992
CGAGGGA11201.9477872E-47.71428542
AGGACGA8500.008155817.62352943
Produced by FastQC (version 0.11.5)