FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005739847

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005739847
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5139543
Sequences flagged as poor quality0
Sequence length150
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATCGGCG1800.064.08
TATCGGC4050.032.07
TCGGCGG4001.8189894E-1225.1999999
TTATCGG5101.8189894E-1221.1764726
ATTATCG8353.905916E-812.07185655
CGATCGG1254.9951684E-410.368130-134
CGTCGCT3002.1827873E-109.615-19
TCGTCGC2405.6601493E-89.59999915-19
CCGATCG1600.004736498.09999955-59
CTCGTCG3052.546767E-78.0262315-19
TCCGGCG3903.6379788E-97.75384625-29
CCGGCGA3952.1278574E-66.56202530-34
CGGCGAG4453.304085E-76.4719130-34
AGTACCT34202.0008883E-116.31578979
CAGTACC29851.3205863E-96.27135668
AAGTCGG7651.8189894E-126.211765140-144
GTTACCG4101.6823142E-45.61951220-24
CCCTAAA49650.05.51057431
CCCAAGT75300.05.45019961
CCAGTAC32651.9719191E-65.0719757
Produced by FastQC (version 0.11.5)