FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005740011

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005740011
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2143609
Sequences flagged as poor quality0
Sequence length150
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATCGGCG650.0110.769238
TCGGCGG1650.048.09
TTATCGG2002.419256E-1036.0000046
TATCGGC2104.0745363E-1034.2857137
ATTATCG3254.2991815E-822.1538475
CTCGTCG1551.9325671E-712.07741815-19
TCGTCGC1551.9325671E-712.07741815-19
CGTCGCT1759.289961E-710.69714315-19
CCAGTAC12851.5513906E-68.404677
CAGTACC13001.8319206E-68.3076938
TTACCGA2252.0513762E-47.6820-24
GTCGCTG3502.2592394E-66.994285620-24
AGTACCT15652.5277037E-56.9009599
GACCTCC14758.675412E-56.8338982
CCGTAAA3451.364343E-56.678260335-39
ACCGTAA3253.0603772E-46.20307735-39
TCCAGTA16553.8683906E-46.09063436
AATCCGG3354.5205536E-46.017910525-29
AGACCTC16150.00190772875.79566571
AACCGCT3854.3608926E-45.6103897105-109