FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005740034

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005740034
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2136736
Sequences flagged as poor quality0
Sequence length150
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATCGGCG801.05501385E-1072.08
TCGGCGG1401.5050318E-841.1428579
TATCGGC1604.8823495E-836.07
TTATCGG2156.511982E-726.7906996
GCGATAT1850.00525481919.459461
GCGAAAG3002.549671E-416.8000011
AGCGAAT2800.003113181315.4285713
CGGGACA3100.00612197813.9354843
TCGTCGC1251.8698302E-713.82399915-19
GCGAGAT3150.0068063113.7142864
TCCGTCA3150.0068063113.7142861
CCGATCG650.00837195813.29230855-59
TTGCGTG3300.0092570113.0909092
ATTATCG3900.001862303112.9230775
GCGAGGA3900.001862303112.9230771
CGTCGCT1354.769081E-712.815-19
CTCGTCG1501.2611781E-712.480000515-19
TCCGGCG1152.3100301E-411.26956625-29
AGAACGA4500.0054360911.21
GCGAGAA4101.6818802E-410.5365861
Produced by FastQC (version 0.11.5)