FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005740036

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005740036
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4120099
Sequences flagged as poor quality0
Sequence length150
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATCGGCG2200.0104.727278
TCGGCGG3850.059.844169
TATCGGC4600.051.652187
TTATCGG4950.046.5454566
ACGCGAA1453.661951E-529.7931024
ATTATCG8800.025.3636365
TCCGGCG3300.016.14545425-29
TCGTCGC2850.014.65263215-19
CGATCGC1201.1358861E-714.40000160-64
CGTCGCT3150.014.17142915-19
CGATCGG1950.014.030769130-134
GCGAAGA5155.347496E-613.9805821
CTCGTCG3400.013.12941215-19
CCGATCG1354.7697904E-712.80000155-59
GCGTTGA4654.9420475E-412.3870971
CGTTGAA7251.1318716E-611.9172422
CCATCGG4250.003549134411.8588231
GCGATCG2201.0004442E-1011.781818130-134
CCGGCGA4250.011.18117730-34
TCGTTAC4200.010.97142815-19
Produced by FastQC (version 0.11.5)