FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005740057

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005740057
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2075480
Sequences flagged as poor quality0
Sequence length150
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATCGGCG700.0102.857148
TCGGCGG1807.8216544E-1140.09
TTATCGG1803.426976E-936.06
TATCGGC2009.586074E-932.3999987
ATTATCG4506.821938E-817.65
TCGTCGC1003.938656E-614.415-19
CTCGTCG1006.273611E-512.95999915-19
CGTCGCT1708.6697337E-48.47058815-19
CCGGCGA2109.2086586E-58.22857130-34
TCCGGCG1600.0047360088.09999925-29
CCGAATC2558.6180976E-46.776470720-24
GGTTCGT2558.6180976E-46.776470715-19
GGGTTCG3354.5205024E-46.4477618
GCTATTA16152.8217855E-46.2414862
AGACCTC15259.593882E-46.1377051
GACCTCC15600.0012602586.02
AATCCGG2950.00443806555.85762725-29
CAGCGGT3854.3608362E-45.610389780-84
AAACCGC3650.00134879875.5232873105-109
GCGAGAA3500.00494299975.348571330-34