FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005740276

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005740276
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1148167
Sequences flagged as poor quality0
Sequence length151
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTGTACG2951.4551915E-1129.5036411
CTAGGAC3800.026.7110213
GACTGCG3554.127287E-922.4642287
ACTGCGC4901.8189894E-1120.7119668
TATACTG7750.020.5801335
GTCCTAC4652.0372681E-1020.2771431
TAGCCCG2959.566302E-619.6605285
TGTACGG1850.005046779319.594962
GCTACAC5254.9112714E-1119.3336933
TAGGACT6000.019.3328514
CTAGACC5107.057679E-1018.4807363
GTATAGG3602.6071211E-618.1324441
TACACTG7450.017.5163425
CTATACT5201.7942511E-816.7303524
GGATATA6104.2382453E-1016.646181
GCGTTAT2700.00232528516.117731
GTGCTAA6306.693881E-1016.117731
TTAGACC4058.03709E-616.1107084
GTACTAT5002.0825428E-715.9565521
CTACACT5554.0017767E-815.6752854
Produced by FastQC (version 0.11.5)