FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005740277

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005740277
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1148167
Sequences flagged as poor quality0
Sequence length151
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG13460.11723033321807716No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGGTCG950.00472839730.5252445
ACCGTAC1409.997544E-425.8919498
TATATCG2857.10162E-620.350165
TACACGG1900.00588960319.0782785
GTACTAC3852.3649045E-718.846921
CTAGGAC4654.50018E-918.709023
TACACCG4705.138645E-918.5099895
GTACGAG2359.203377E-418.5099893
ACTAGAC2450.001217360817.7544783
TACACTG6950.017.7331895
TAACCTC5351.3369572E-917.6162035
GTCTTAC5101.396802E-817.0730931
GTAGACT3002.4179112E-416.9160733
CGTACAC2650.002058496516.4145183
GTCTAGT2700.002318689116.1245881
GAACCGT4501.2232722E-616.1105466
CTATACT5402.8570867E-816.1105444
GGACCGT2700.002332218216.1105446
AGTACGG2700.002332218216.1105445
CATACAC8650.015.9242953
Produced by FastQC (version 0.11.5)