FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005740278

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005740278
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8354951
Sequences flagged as poor quality0
Sequence length151
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTATACT47000.013.7284334
GTGTAGG53950.013.1731071
TATACTG67600.013.0840425
GTATAGG28100.012.9037891
CTAATAC45850.012.6496773
CTACACT47650.012.4761254
GTCCTAC28800.012.3383521
CCTATAC25400.012.2733623
TCTACAC51700.011.9194763
GTATATG71300.011.9000811
TCCGTAC9751.9972504E-911.8968278
TCTATAC43350.011.7067883
CTAGGAC34850.011.6496823
GTGTAGA54200.011.2391531
GTATAGA51100.011.2113981
CTACACG18200.011.1536094
GTCCTAG35700.010.9693051
GTATTAG38550.010.9108141
TAGTACT56100.010.8553854
GTGCTAG38150.010.8351231
Produced by FastQC (version 0.11.5)