FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005740279

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005740279
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8354951
Sequences flagged as poor quality0
Sequence length151
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTACACT45850.015.654224
TATACTG70350.014.4277815
GTATAGG26800.013.7998691
CTAGCAC35800.013.7708723
GCGTTAG6903.8604412E-813.6626151
GTAGGAC32600.013.5658753
GTGTAGC41600.013.0739711
TCTAGAC41100.012.8770573
TAGCACT64200.012.6479044
TACACTG65050.012.4826365
ATACGCC7006.8145164E-712.4285033
TAGACCG5851.8526483E-512.3930935
GTATACG13000.012.27209951
TATACCG7701.6235572E-712.2401915
GTGCTAG37950.012.2294721
TACACCG17250.012.1883385
GTATAGA47950.012.09876251
TAGGACT52950.011.6382734
GTGTAAG57700.011.3111371
GTACTAG33500.011.2563631
Produced by FastQC (version 0.11.5)