FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005740283

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005740283
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10645791
Sequences flagged as poor quality0
Sequence length151
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTACACT59100.013.861864
GTGTAGG80650.013.8553741
GTGCTAG49150.013.7297111
TACACTG78750.013.6251635
TATACTG77500.013.4707365
GTATAGC35850.013.1560691
ACATACG13700.012.70093145
TATACGC8755.1059033E-912.4283582
TATACCG10303.7107384E-1011.9658155
ATACGCC9751.9954314E-911.8972323
TAGGGGT55500.011.7565554
GTATACG16100.011.7178891
CCTACAC68700.011.7137733
GTATAGA56250.011.6097251
GTATAAG56800.011.4973071
ATCGTAC8959.1948095E-811.3502891
CTAGGAC41600.011.327933
GTATAGG32050.011.3199351
CTATACT53950.011.2880364
GTGTATG101050.011.2736691
Produced by FastQC (version 0.11.5)