FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005740288

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005740288
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5862273
Sequences flagged as poor quality0
Sequence length151
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGACCG3956.347507E-616.5183665
GTATAGG17200.013.4932751
GCTATAC17900.013.3655353
CTAGCAC24550.013.288823
TCTAGAC28500.012.9733093
GTGTAGG39050.012.8151561
GTACTCG7501.1465818E-712.5712351
TACACTG50100.012.4446435
GTAGGAC21000.012.4282113
CCGCACT8906.5738277E-912.2186424
ATAGCCG4856.647961E-411.9582128
GCGTTAC4250.0033612111.9455181
CTAGACT23650.011.6486124
CTACGTT9401.4773832E-811.5687144
TACGGTG8455.4195516E-711.1534285
TACACCG18850.011.1534275
CTAGCCT32700.011.0852124
CTAAGAC23000.011.03228953
TAGGACT34950.010.9938654
GTAGGGC22050.010.8500263
Produced by FastQC (version 0.11.5)