FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005740289

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005740289
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5862273
Sequences flagged as poor quality0
Sequence length151
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACCGTC5253.6482925E-715.1902617
TACACCG20000.014.8622895
CTACACT31750.013.7005934
GTACGTC5458.938689E-613.3025643
ACATACG8854.311005E-1013.10783145
CTATACC20150.012.952674
TACACTG47350.012.7083725
GTATAGA31300.012.5149611
GTCTTAG23400.012.4000731
CCTACAC39800.012.3867593
TCTATAC28850.012.3135173
GTATAGG17750.012.2603541
CCTATAC16050.012.1960893
GTGTTAG38350.012.1058341
AAGACCG11802.3646862E-1111.6735645
CTATACT28800.011.3279644
GTCTTAC31500.011.2840661
CTAGGCT28350.011.2520454
GTCTAAC16900.011.1600651
CTATACG7201.261495E-511.0762314
Produced by FastQC (version 0.11.5)