FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005740291

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005740291
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2347982
Sequences flagged as poor quality0
Sequence length151
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGTTAG2502.2655822E-623.2185761
GTGTACG4451.1277734E-1021.1967331
GTAGGAC9900.016.8432793
GTATTAG9600.015.8720721
CTACACT11250.015.4665234
GTGCTAG9550.015.1954021
CGTACAC4101.6067694E-414.146213
GTTAGCC7707.548806E-1014.1232473
TTATACG3700.001188972713.7160884
AACGCTA3200.00721652213.5936235
CCTACAC13550.013.376263
TACACGG5551.0774238E-513.06294255
TACGACA5056.4507185E-512.9206724
CTACACG4553.8804478E-412.7471344
ATAGGAC10855.456968E-1212.6957353
TACACTA11451.8189894E-1212.6636385
TAGGACT13850.012.5630614
TAGGACA15050.012.5248014
GTCTAGA9351.0168151E-912.4163491
GTATATG21800.012.3148981
Produced by FastQC (version 0.11.5)