FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005740293

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005740293
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3673075
Sequences flagged as poor quality0
Sequence length151
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTACGGT2650.002059249716.4150056
GTATAGG11600.015.6359821
CTACACT21850.015.5948784
TACACTG27100.015.516525
CGCCGTA7303.3833203E-1014.897381145
GTGCTAG14350.014.6618651
CCGCACT5959.4143616E-814.6217684
CTACACG9253.6379788E-1214.108034
CCTACAC21550.013.7934273
ATAGCGC4453.2171284E-413.0336363
GTGTATA25250.012.6425431
TATACTG30750.012.4958655
CCTATAC11057.2759576E-1212.4659943
AATTGCG4200.003084385312.0832677
ACGAGTC4806.09041E-412.0832668
GTACTAT19250.012.0604181
GCTATAC12650.012.0355063
CTAATAC16450.011.899633
TATACAG33850.011.7798455
ACTACAC19900.011.6582273
Produced by FastQC (version 0.11.5)