FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005740294

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005740294
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7174230
Sequences flagged as poor quality0
Sequence length151
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATAGG21150.017.4898821
GTACGTC5552.1846063E-916.9820333
GTGTACG10900.016.635681
GTGTAGG50000.014.5063131
TAGACCG5005.88079E-513.0495865
GTATAGA39100.012.9851911
TATGCGT6202.3807534E-612.86249354
GTGCTAG34450.012.6324931
CTAGCAC29450.012.5552173
GTATTAG27350.012.1990941
CTACACT37700.012.1154
TATACTG50650.012.0233115
GTATATA67450.011.8287211
ACATACG6153.094833E-511.787668145
TCTATAC36700.011.6553493
CTAATAC32000.011.5547243
GCGTAGA10053.1905074E-911.5473141
TATACGC5701.9961601E-411.4481222
CTATACT36900.011.3953024
GTCTAGA31300.011.3547821
Produced by FastQC (version 0.11.5)