FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005740295

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005740295
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7174230
Sequences flagged as poor quality0
Sequence length151
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG72560.10113977388514167No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATAAG39000.014.6969521
TAGGACT43050.013.6410134
GTACTAG25600.013.3205661
GTGCTAG31050.013.3191981
GTATATA70300.013.2105061
TACACTG55450.012.9439975
CCTATAC19700.012.8806143
CTACACT37950.012.7996414
GTATAGA37450.012.5929171
GTTTAGG53000.012.4574841
TAGACCG4806.091757E-412.0832435
TCTAGAC33650.012.06528953
TAGCACT50450.011.9275624
CTAGTAC24550.011.8125383
GTCTAGA27300.011.6937781
CTAGCAC31400.011.5444993
CTAATAC30850.011.5153113
GTATAGC20800.011.5110631
TATGCGT6303.955891E-511.507854
GTATGCG6455.028841E-511.2402263
Produced by FastQC (version 0.11.5)