FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005740297

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005740297
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5215767
Sequences flagged as poor quality0
Sequence length151
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATAGG14900.018.5053751
TATACTG41150.017.0897565
TAGGGGT22650.015.6841824
TACACTG41550.014.831395
CTACACT28900.014.0483124
CTGTCCG12500.013.3398769
TAGTCGG4352.654972E-413.333215
TACGTCT9306.730261E-1113.2525654
GTTTAGG36300.013.1928261
GTATAAG32150.012.8645441
GTACCGT4553.881862E-412.7471346
AACCGCG5208.487963E-512.547967
GTATAGA29200.012.424751
CATACCG5851.8524026E-512.3930475
CTAGTAC17650.012.3228323
CGTATGG5301.0141789E-412.3112073
CTAGCAC20700.012.2583413
GTATTAC37300.012.2555341
TAGGACT30250.012.2230274
TTATACT52200.012.2221094
Produced by FastQC (version 0.11.5)