FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005740298

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005740298
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences302379
Sequences flagged as poor quality0
Sequence length151
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGCTCG350.003564258662.142154
GTTAGAC750.001481831138.6662253
TTAGACT951.027259E-438.157464
GTTACAC1458.672523E-734.99963
CTACCCT950.004722399630.5259674
GTATTAG1000.00605348729.0140651
GTCCTCG1000.006068185428.9996689
GTACCCC1701.029043E-425.5879444
ATGCACC1450.001224546124.9997144
GTATATA1550.001800498123.3984381
CAAGACC1902.1884253E-422.8944744
TATGCTC1600.0021718122.6559935
GTACAGG2003.0859807E-421.7605481
TAACTCC1700.003088069121.3232864
GTACAGA2053.6463133E-421.2298031
ATGATCG2404.3503416E-521.1455925
ACAGGTC1800.004300524520.1386598
CCTTGCG2205.890758E-419.7725012
GAGGCGT2205.890758E-419.7725016
GGCCTAC1850.0050247819.6040951
Produced by FastQC (version 0.11.5)