FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005740301

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005740301
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9225388
Sequences flagged as poor quality0
Sequence length151
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG95280.10328020891912622No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATAGG29200.014.6601041
CCTATAC26050.014.1936343
GTAGGAC38500.013.7465363
TATACTG75950.013.5547565
CCTAACG4955.3509983E-513.1816812
TACACTG73950.013.1370485
GTATAGA55550.012.7999971
GTAACGC6252.6048565E-612.7597983
GTATAGC29700.012.7032471
GTGTAGG70350.012.5824031
CTATACT51550.012.23554
GTGCTAG41050.012.1956181
TACACCG22650.012.1631625
CCTACAC55700.012.10483553
CTACACT52600.011.7155954
GCTATAC29050.011.4800253
CTAGGAC34300.011.4138143
TAGGACA70000.011.3926774
GTCCTAG38400.011.3367331
GTATTAG39200.011.290461
Produced by FastQC (version 0.11.5)