FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005740302

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005740302
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7962025
Sequences flagged as poor quality0
Sequence length151
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACACTG65050.015.2683765
CTACACT44800.014.7259374
TATACTG61900.013.8200935
TAGACCG5801.1252032E-613.7494165
CCTATAC25150.013.5481323
CCTACAC44750.013.4463573
TATACGC8252.1245796E-913.1812595
TACGTCG3300.00884877713.1812585
GTGCTAG35800.013.1683071
GTATAGG25950.013.1359081
TCTATAC40950.013.1007873
CTTACAC57000.012.5915713
GTAGGAC34300.012.4703173
TACACTC39550.012.4647055
TAGGACT49450.012.4615544
GTGTAGG45450.012.4468751
GTATTAG32350.012.3307081
TATACAG70000.012.3244765
GTCTAGA35000.012.2259781
CTACCCT48750.011.74821954
Produced by FastQC (version 0.11.5)