FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005740303

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005740303
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7962025
Sequences flagged as poor quality0
Sequence length151
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG82800.10399364483281578No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATAGG26200.017.1684931
CTAGTAC26200.014.9424983
TCTAGAC36950.014.9117953
CTATACT43700.014.2674964
CTACACT43450.013.6821654
TACACTG65250.013.4442215
GTAACGC5005.880122E-513.0497813
TATACTG64150.012.883655
GTCTAGT23750.012.8300231
GTATAGA43800.012.7543511
ATACGCC6855.241927E-712.7005183
GTCTAGA35600.012.4314441
GTATTAG32250.012.37299251
TCTATAC40900.012.2308473
ACACCGC17850.012.18466956
TACACTA41900.012.1119695
TAGACTG44500.011.7301415
GTATAGC28050.011.6391541
CCTACGC11351.5279511E-1011.4976053
CCTATAC27950.011.4130483
Produced by FastQC (version 0.11.5)