FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005740305

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005740305
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6062974
Sequences flagged as poor quality0
Sequence length151
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTACCG4506.312075E-817.7215185
GTATAGG19050.015.2349311
TAGCACG8802.7284841E-1114.0051254
CTACACT30200.013.6832314
TATACTG44250.013.5983295
CCTATAC17750.013.4783383
GTATACG10451.8189894E-1213.1920641
TAACGCA5005.8809386E-513.0494814
TATACGC6252.6051366E-612.7594932
TACACTG46150.012.7243045
CTATACT30050.012.54525854
GTATAGA30000.012.334581
CTATACA33300.011.9739984
TAGCACT44350.011.9330094
CCGCTAG5551.5591906E-411.75628959
GCTATAC17750.011.4361663
GTATATG44850.011.3242911
TCTATAC31400.011.3132443
TAGGGGT27150.011.2150244
GCCGTAC6555.8346464E-511.0773061
Produced by FastQC (version 0.11.5)