FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005740306

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005740306
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10782996
Sequences flagged as poor quality0
Sequence length151
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATACGCC10850.016.0361823
CTATACT59250.014.68271354
CCTACAC68250.014.4462713
CTACACT59500.014.3773394
GTATAGG30450.013.575761
TACACTG87750.013.5490915
TAACGCT13450.013.4750754
TATACTG84400.013.3997165
GTGTACG18200.013.14981
GCTATAC34950.013.0681133
GTGCTAG48150.012.6520131
TATACGC10353.092282E-1112.6083342
TAGGGGT51800.012.5958144
CTAGCAC43800.012.5793733
TACTCCG18300.012.2807265
GTGTAGA66400.012.1235981
GTATAGC34850.011.8617491
CTAGACT42150.011.6956624
GTATAGA63300.011.6861931
GTACTAT49650.011.6855021
Produced by FastQC (version 0.11.5)