FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005740309

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005740309
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9936452
Sequences flagged as poor quality0
Sequence length151
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTACACT57250.014.9430434
TACACTG81100.014.4819385
TAGACCG5751.0203421E-613.869395
GTATTAG36050.013.0826231
GCTATAC32850.013.0211453
GTGCTAG43050.012.9778951
CTATACT53000.012.3111654
TAGGACT62800.012.2371074
GTATAGG32350.012.1117311
GTACTAG36300.011.9930961
TACACCG21200.011.9691895
CTAATAC45650.011.9111313
TATACTG76550.011.743815
GTATAAG55400.011.6564671
GTATAGC34350.011.6177661
GTGTAGG72050.011.58112051
CCTACAC61350.011.5809473
CTAGGAC40900.011.521863
GTATACG16600.011.3645431
TAGACTG55800.011.1736945
Produced by FastQC (version 0.11.5)