FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005740310

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005740310
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7943782
Sequences flagged as poor quality0
Sequence length151
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATACTG62300.014.080485
TATACGC7406.555638E-913.715655
CTAATAC37800.013.4254563
GTGTAGA50550.013.2006271
TAACGCA7156.175651E-813.1812744
TACGCTG16500.013.1812745
ATAACGC7451.05645086E-712.6505653
TAACGCT8957.1449904E-912.1503374
GTAACGC5451.316316E-411.9720583
GTATAGG27350.011.9339181
TACACTG66850.011.9292025
GTATATG66300.011.705721
GTGTACG13900.011.4798391
TAGCACT59450.011.3409964
GTCTAGA38400.011.3330781
TAGGACT48650.011.3253294
CTAGACT32650.011.3241884
GTGTAGG51350.011.2999741
CTACACT45900.011.2141344
TACACCG18150.011.1841125
Produced by FastQC (version 0.11.5)