FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005740313

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005740313
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7773224
Sequences flagged as poor quality0
Sequence length151
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTGTACG10850.014.7060841
TATACTG65250.014.111065
CCTATAC23400.013.9422593
GTATAGG23100.013.8148071
GTGTAGG57950.013.6419731
ATACGCC6603.3369724E-713.1817713
CTACACT43900.012.5512084
GTGCTAG33800.012.4455881
CTAGCAC32250.012.3642973
GTAGGAC32300.012.3451583
GTATAGA43000.012.3128471
GTACTAG29100.012.2125741
TACACTG63400.012.12140755
TTATACG9651.6952981E-912.0206834
CTAGGAC31400.012.0063273
TTAGGCG4856.645299E-411.958875145
GTACGTC6153.092475E-511.7885763
TAGCACT58650.011.743354
TGTACGG6907.862805E-611.557932
TAACACT84700.011.5554494
Produced by FastQC (version 0.11.5)