FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005740315

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005740315
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7500980
Sequences flagged as poor quality0
Sequence length151
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG82510.10999896013587558No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTATACT39350.018.6082024
CCTATAC22500.016.7551613
GCTATAC22600.016.6810253
CTACACT42600.016.6780134
TATACTG57350.015.422485
TACACTG56800.015.1889045
CTATACA41100.014.9935664
GTAGGAC30100.014.6923463
TATACCG7851.0095391E-913.8531785
CTAGGAC30900.013.8427173
TCTATAC37600.013.6898383
TAGGACT47450.013.4454164
ACTATAC31900.012.9542413
GCGTTAC5157.703755E-512.6788181
TACACCG18300.012.6772985
TCTACAC46950.012.6621083
GTACTAG27700.012.5720161
GTCTTAG32250.012.373041
GTGTATA57250.012.2924861
TGTACTG69700.012.1697295
Produced by FastQC (version 0.11.5)