FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005740316

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005740316
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1378057
Sequences flagged as poor quality0
Sequence length151
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATAGG3351.3111403E-619.4838181
GTCATAC4954.7293724E-1019.046471
GGGTAGA6503.6379788E-1217.851841
GCGTTAG2500.001390784917.4055441
GTGTAGA7200.017.123511
GTATAGA7301.8189894E-1216.8889431
TCTATAC6154.783942E-1016.5033343
CGTAACA2750.002636521115.8174812
GGCCTAT4852.6593316E-614.9532181
GTATTAC9250.014.8966381
TATACTG8151.1641532E-1014.2324985
GTATATA12050.013.8425841
GGTTAGC4302.3987387E-413.492671
CCTATCC4302.4061237E-413.4877753
TAGTGCA5951.4972902E-613.4027679
GTGTACG3250.00797622213.388881
ATAGTAC6001.6450049E-613.2910783
CTATCCG3300.00884402113.1812344
TAACACG3300.00884402113.1812344
TCCGTCC4453.2159057E-413.0331328
Produced by FastQC (version 0.11.5)