FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005740318

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005740318
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6915178
Sequences flagged as poor quality0
Sequence length151
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATACG11050.017.7212771
GTATAGG21350.015.6262041
GTGCTAG30550.015.4310581
TATACGC7053.3123797E-914.3974252
GTGTAGA43900.013.5469921
GTATAGC22100.013.4550441
GTGTAGG44300.013.2609561
TAGGACT42650.012.7486154
GTATAGA39550.012.6530771
CTAGGAC28150.012.6194843
TACACTG54050.012.2058065
TACACCG15550.012.1217055
GGCGTAT5401.2034598E-412.0876621
TAGAGCG8454.1896783E-812.0113584
GTACTAG24950.011.9180961
ACATACG8554.9363734E-811.87053145
GTATTAG26450.011.7906111
GTGTACG11101.0186341E-1011.7609671
ATACGCC6203.360243E-511.693173
CTACACT36600.011.6866954
Produced by FastQC (version 0.11.5)