FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005740369

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005740369
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12749719
Sequences flagged as poor quality0
Sequence length65
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACAACGTGATATCTCGTATGCCGTCTTCTGCTTG283610.22244411818017323TruSeq Adapter, Index 1 (97% over 36bp)
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA215010.16863901078917895No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATGCCG37600.050.6046248
TCGTATG38400.049.62698745
CGTATGC40200.047.33205846
TATCTCG40400.047.09718741
CTCGTAT40150.047.0961844
ACGTGAT42300.045.0498635
CGTGATA44100.043.4117636
AACGTGA45050.042.10359634
CACAACG45800.041.54326631
CAACGTG47150.040.4786133
GTATGCC48800.039.1118147
ATGCCGT50800.037.4553949
GCCGTCT52250.037.0371551
TCTCGTA55350.034.69604543
ACAACGT55250.034.33075732
GATATCT57400.033.353239
TGCCGTC60750.031.56351950
ATATCTC60900.031.43659440
CGTCTGA61450.031.2987516
GTGATAT62050.031.2815937
Produced by FastQC (version 0.11.5)