FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005740371

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005740371
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences16684255
Sequences flagged as poor quality0
Sequence length65
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA526300.31544710866622455No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACAAACATCGATCTCGTATGCCGTCTTCTGCTTG303100.1816682854583558TruSeq Adapter, Index 5 (97% over 36bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATGCCG38800.046.3026848
TCGTATG39050.045.9302945
CGTATGC42400.042.4410346
ATCGATC41900.042.3123138
CTCGTAT42800.041.90577744
AACATCG50250.035.8680335
GTATGCC53000.034.28698747
CATCGAT57100.031.56540537
TCTCGTA57800.031.4901943
GCCGTCT59650.030.9093751
ATGCCGT59350.030.61826349
ACATCGA63750.028.31871636
CGTCTGA66150.027.64802716
ACGTCTG68950.026.26857415
TGCCGTC70450.026.04533650
ACACGTC72050.025.22038513
GATCTCG72300.025.21553441
ATCTCGT71800.025.14461142
CACGTCT78200.023.38768814
TCGATCT76950.023.30779639
Produced by FastQC (version 0.11.5)