FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005740372

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005740372
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences16625253
Sequences flagged as poor quality0
Sequence length65
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA530860.3193094264490291No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACAAACATCGATCTCGTATGCCGTCTTCTGCTTG296480.17833112073542579TruSeq Adapter, Index 5 (97% over 36bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATGCCG37850.048.47702848
TCGTATG39950.045.8549645
CGTATGC41550.043.9471946
ATCGATC41450.043.62620538
CTCGTAT42600.043.14097644
AACATCG48300.037.37797535
GTATGCC51600.035.61638647
CATCGAT56300.032.27630637
GCCGTCT59250.031.46590651
ATGCCGT59100.031.14645449
TCTCGTA60000.031.12174643
ACATCGA63500.028.89536336
TGCCGTC69800.026.6254350
CGTCTGA70500.026.23553516
ACGTCTG71000.026.1754215
GATCTCG73150.025.20444741
ACACGTC73850.025.08537713
ATCTCGT74100.024.92112442
TCGATCT73350.024.85420439
GTCACAA76400.024.40342929
Produced by FastQC (version 0.11.5)